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Identification of Functional Protein Regions Through Chimeric Protein Construction
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JoVE Journal Bioquímica
Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

DOI:
10.3791/58786-v

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11:39 min

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January 08, 2019

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, , ,
1Department of Cardiac Development and Remodelling,Max Planck Institute for Heart and Lung Research, 2Partner site Rhein-Main,German Centre for Cardiovascular Research (DZHK)

Capítulos

  • 00:04Título
  • 00:49Chimeric Protein Design
  • 05:00Preparation for Molecular Cloning
  • 07:05Polymerase Chain Reaction (PCR) Amplification of the Individual DNA Fragments Forming the Chimera and to Generate the Chimeric DNA Sequence
  • 09:22Results: Generation of the Oncostatin M BC Loop Chimera
  • 10:55Conclusion

Summary

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Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence.

Tags

Protein ChimeraFunctional Protein RegionsProtein StructureProtein SequenceProtein EngineeringBiologia MolecularStructural BiologyProtein Domain ExchangeProtein Domain Swapping

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