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Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

DOI:
10.3791/58792-v

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08:21 min

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April 01, 2019

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, , , , ,
1Charles R. Allen Research Laboratories, Department of Anesthesiology,University of Texas Medical Branch, 2The Moody Project for Translational Traumatic Brain Injury Research,University of Texas Medical Branch

Capítulos

  • 00:04Título
  • 01:06Advanced Blast Simulator (ABS) Blast Device Preparation
  • 02:06ABS Injury Preparation
  • 03:58Blast-traumatic Brain Injury (TBI) Induction
  • 04:38Rodent Middle Cerebral Artery (MCA) Segment Extraction
  • 07:04Results: Representative Effects of Blast-induced Traumatic Brain Injury (bTBI) on MCA Responses to Reduced Intravascular Pressure
  • 08:00Conclusion

Summary

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Here, we present a protocol to describe methods for ex vivo vascular reactivity determination following a primary blast traumatic brain injury (bTBI) using isolated, pressurized, rodent middle cerebral arterial (MCA) segments. bTBI induction is accomplished using a shock tube, also known as an Advanced Blast Simulator (ABS) device.

Tags

Keywords: Blast-induced NeurotraumaPressurized Rodent Middle Cerebral ArteriesCerebral Vascular InjuryMyogenic Vascular ResponseIsolated Pressurized Arterial PreparationAdvanced Blast SimulatorMylar SheetsHydraulic Hand PumpAnesthetized RatEndotracheal TubeVentilatorSpecimen TrayHeat LampScalpEar Plug

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