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cd8+ t 细胞诱导体外肿瘤细胞凋亡的实时检测--研究肿瘤浸润髓内细胞的免疫抑制作用
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
DOI:

09:57 min

January 29, 2019

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Capítulos

  • 00:04Título
  • 00:59Isolated CD8+ T Cell Activation and Expansion
  • 01:49Pre-Activated CD8+ T Cell:Target Cell Co-Culture
  • 04:49Cell Imaging and Analysis
  • 07:39Results: Representative Effector CD8+ T Cell and Target Cancer Cell Interactions
  • 09:06Conclusion

Summary

Tadução automática

我们在这里描述了一个方案,研究细胞的预激活 cd8+ t 细胞对癌细胞通过实时显微镜检测凋亡癌细胞。该方案可以研究骨髓细胞诱导的 t 细胞抑制背后的机制, 并评估旨在通过阻断免疫抑制骨髓细胞来补充 t 细胞的化合物。

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