Journal
/
Imunologia e Infecção
/
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

DOI:
10.3791/58845-v

•

09:57 min

•

March 14, 2019

•

, , , , , ,
1The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics,Guangzhou Women and Children’s Medical Center, 2CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai,Chinese Academy of Sciences, 3CAS Key Laboratory of Infection and Immunity (CASKLII), Institute of Biophysics,Chinese Academy of Sciences, 4Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences

Capítulos

  • 00:04Título
  • 00:31Virus Propagation and Collection
  • 02:10Infective Dose Determination
  • 03:48Wolbachia-Free Fly Preparation and Infection
  • 08:00Results: Representative Host-Virus Interactions in DCV-Infected Drosophila
  • 08:47Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes how to establish viral infection in vivo de Drosophila melanogaster using the nano-injection method and basic techniques to analyze virus-host interaction.

Tags

Keywords: Viral InfectionDrosophila MelanogasterHost-virus InteractionNanoinjectionVirus-host InteractionS2 Star CellsDrosophila C VirusDCVCell MorphologyCell DebrisVirus ConcentrationVirus Dilution

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58845?article_type=v

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
check_url/pt/58845?article_type=v

Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster
check_url/pt/58845?article_type=v

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
check_url/pt/58845?article_type=v

Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
check_url/pt/58845?article_type=v

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
check_url/pt/58845?article_type=v

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
check_url/pt/58845?article_type=v

Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster
check_url/pt/58845?article_type=v

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay
check_url/pt/58845?article_type=v

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
check_url/pt/58845?article_type=v

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Read Article