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Bio-energetik undersøgelse af Candida albicans ved hjælp af Real-time ekstracellulære Flux analyse
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JoVE Journal Medicina
Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis

Bio-energetik undersøgelse af Candida albicans ved hjælp af Real-time ekstracellulære Flux analyse

DOI:
10.3791/58913-v

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08:48 min

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March 19, 2019

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, , ,
1Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers,The State University of New Jersey, 2Public Health Research Institute, Rutgers,The State University of New Jersey

Capítulos

  • 00:04Título
  • 00:48Coating of the Assay Plate with Poly-D-Lysine
  • 01:53Hydration of Sensor Cartridge
  • 02:33Growing and Seeding Cells in the Poly-D-Lysine-coated Plates
  • 03:33Assay Protocol
  • 05:56Results: Real-time Extracellular Flux Analysis
  • 07:59Conclusion

Summary

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Tadução automática

Vi præsenterer her, en trinvis protokol for at undersøge den mitokondrielle respiration og glycolytic funktion i Candida Albicans ved hjælp af en ekstra flux analyzer.

Tags

Keywords: Candida AlbicansBio-energeticsExtracellular Flux AnalysisMitochondrial RespirationGlycolytic FunctionFungal PathogensPoly-D-LysineSensor CartridgeYPD BrothAssay Medium

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