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免疫荧光定量小鼠脑突触蛋白的异种分布

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

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免疫荧光定量小鼠脑突触蛋白的异种分布

DOI:

10.3791/58940-v

•

09:18 min

•

January 29, 2019

•

Rebecca Wallrafen, Thomas Dresbach, Julio S. Viotti

¹Institute of Anatomy and Embryology,University Medical Center Göttingen

Capítulos

00:04Título
00:55Sample Preparation
02:28Immunofluorescence
06:08Analysis of Confocal Images
07:11Results: Determining the Abundance of Proteins of Interest Across the Brain
08:43Conclusion

Summary

Tadução automática

在这里, 我们描述了一种定量的方法来确定突触蛋白相对于标记蛋白使用免疫荧光染色, 共聚焦显微镜, 和基于计算机的分析。

Tags

Keywords: Immunofluorescence Confocal Microscopy Protein Distribution Heterogeneity Mouse Brain Cryo-protection Cryosectioning Brain Slices Immunostaining Quantification

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