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JoVE Journal Neuroscience
Identifiera cell ytan markörer av primära neurala stamceller och stamceller genom metabolisk märkning av Sialoglycan
 
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Identifiera cell ytan markörer av primära neurala stamceller och stamceller genom metabolisk märkning av Sialoglycan

Article DOI: 10.3791/58945-v • 11:39 min • September 7th, 2019
September 7th, 2019 •
*1, *2, 1
1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Presenteras här är ett protokoll som kombinerar en in vitro -neural-endotelial Co-Culture system och metabolisk inkorporering av sialoglycan med bioorthogonal funktionella grupper för att expandera primära neurala stamceller och stamceller och märka deras yta sialoglykoproteiner för avbildning eller masspektrometri analys av cell ytan markörer.

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Neurovetenskap metabolisk glykananalys märkning sialic syror bioorthogonal reaktion neurala stamceller stamceller ytmarkörer endotelceller Proteome kemisk märkning hjärnbarken
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