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Un protocole en acier inoxydable pour l’ARN haute qualité laser capturer des cellules de Microdissected Purkinje du cervelet humain Post-Mortem
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1Department of Pathology and Cell Biology,Columbia University, 2Division of Movement Disorders, Department of Neurology,Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health,Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine,Yale University
Capítulos
- 00:04Título
- 00:33Prepare Cryostat for Tissue Sectioning
- 01:51Sectioning Tissue
- 03:50Fixing Tissue/Stain-less Visualization
- 04:47Laser Capture Microdissection of Purkinje Cells
- 06:09Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
- 07:59Conclusion
Ce protocole utilise une approche sans tache de visualiser et d’isoler les cellules de Purkinje dans les tissus frais congelés de cervelet humain post-mortem par microdissection de saisie de laser. Ce protocole vise à produire des quantités suffisantes d’ARN de haute qualité pour RNA-sequencing.
