/
/
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Título
- 00:32Cell Preparation
- 02:15Cell Staining – General Procedure
- 04:02Antibody Titration
- 06:41Gating Strategy
- 08:15Results: Analysis of Samples from Patients with Multiple Myeloma
- 10:02Conclusion
Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.
