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An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
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Biologia
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JoVE Journal Biologia
An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

DOI:
10.3791/58966-v

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09:47 min

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February 01, 2019

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,
1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences,Yonsei University

Capítulos

  • 00:04Título
  • 01:05Generation of LN215-YFPQL and LN215-I” Cells by Lentiviral Transduction
  • 03:14Plating the LN215-YFPQL and LN215-I‐ Cells
  • 04:42Conducting the I-YFP GJIC Assay
  • 07:37Results: Analysis of the Iodine-Yellow Fluorescent Protein-Gap Junction Intercellular Communication Assay
  • 08:15Conclusion

Summary

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Tadução automática

Here, we present a protocol for a novel gap junction intercellular communication assay designed for the high-throughput screening of gap junction-modulating chemicals for drug discovery and toxicological assessment.

Tags

Keywords: Iodide-YFPGap JunctionIntercellular CommunicationHigh-throughput ScreeningAssayLN215 CellsLentivirusPuromycinCell Culture

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