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超薄聚二甲基硅氧烷微氟化片在保水作用下固定化活线虫个体

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

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超薄聚二甲基硅氧烷微氟化片在保水作用下固定化活线虫个体

DOI:

10.3791/59008-v

•

04:55 min

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March 19, 2019

•

Michiyo Suzuki, Tetsuya Sakashita, Tomoo Funayama

¹Department of Radiation-Applied Biology Research, Takasaki Advanced Radiation Research Institute,National Institutes for Quantum and Radiological Science and Technology (QST-Takasaki)

Capítulos

00:04Título
00:45On-Chip Immobilization
02:22Collection of Animals from a Worm Sheet
02:53Application of Worm Sheets for Imaging Observations
03:11Treatment of Worm Sheets for Repeated Use
03:52Results: C. elegans Individuals Enclosed with PS Film Display No Reduction in Motility
04:20Conclusion

Summary

Tadução automática

建立了一系列固定化方法, 允许使用最近开发的具有水保留的超薄聚二甲基硅氧烷微流体芯片对活的虫进行定向照射。这种新颖的片上固定也足以进行成像观测。详细说明了该芯片的处理和应用实例。

Tags

Keywords: Caenorhabditis Elegans Microfluidic Chip Worm Sheet Water Retention Immobilization Microbeam Irradiation Behavioral Assays Long-term Observation Polydimethylsiloxane Cover Film Buffer Solution Platina Picker Culture Plate Stereomicroscope

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