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在生物安全2级环境中制备假型颗粒研究高致病性冠状病毒
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
DOI:

08:40 min

March 01, 2019

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Capítulos

  • 00:04Título
  • 00:41Cell Seeding for Pseudotyped Particle Production
  • 02:05Three-plasmid Co-transfection
  • 03:53Pseudotyped Particles Collection
  • 04:46Pseudotyped Particle Infection
  • 05:54Infectivity Quantification by Luciferase Assay Readout
  • 07:04Results: Infectivity Assays of SARS-CoV S and MERS-CoV S Pseudotyped Particles
  • 08:02Conclusion

Summary

Tadução automática

在这里, 我们提出了一个协议, 以产生伪型粒子在 bsl-2 设置纳入高致病性病毒中东呼吸综合征和严重急性呼吸综合征冠状病毒的尖峰蛋白。这些伪类型粒子包含一个荧光素酶报告基因, 允许病毒进入目标宿主细胞的定量。

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