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Produksjon av Pseudotyped partikler for å studere svært patogene Coronaviruses i en Biosafety nivå 2
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1Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering,Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, 5Horae Gene Therapy Center,University of Massachusetts Medical School
Capítulos
- 00:04Título
- 00:41Cell Seeding for Pseudotyped Particle Production
- 02:05Three-plasmid Co-transfection
- 03:53Pseudotyped Particles Collection
- 04:46Pseudotyped Particle Infection
- 05:54Infectivity Quantification by Luciferase Assay Readout
- 07:04Results: Infectivity Assays of SARS-CoV S and MERS-CoV S Pseudotyped Particles
- 08:02Conclusion
Her presenterer vi en protokoll for å generere pseudotyped partikler i BSL-2 omgivelser omfatter spike protein svært patogene virus Midtøsten åndedretts syndrom og alvorlig akutt åndedretts syndrom coronaviruses. Disse pseudotyped partikler inneholder et luciferase reporter gen slik at kvantifisering av viruset inn i vert målcellene.
