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An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
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JoVE Journal Medicina
An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay

An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay

DOI:
10.3791/59025-v

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09:14 min

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January 26, 2019

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, , , , , , ,
1Murdoch Children’s Research Institute, 2Department of Paediatrics,University of Melbourne, 3School of Chemistry and Molecular Bioscience,University of Queensland, 4London School of Hygiene and Tropical Medicine

Capítulos

  • 00:04Título
  • 00:48Assay Setup
  • 05:23Assay Development
  • 07:40Results: A High-throughput Image-based RSV Neutralization Assay
  • 08:43Conclusion

Summary

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Tadução automática

This study describes a high throughput, imaging-based micro-neutralization assay to determine the titer of neutralizing antibodies specific for respiratory syncytial virus (RSV). This assay format has been tested on different sample types.

Tags

Keywords: Respiratory Syncytial VirusRSVMicro-neutralization AssayNeutralizing AntibodiesHigh ThroughputImaging-basedA549 CellsSerum DilutionReference SerumSample Preparation

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