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基于逆电子需求函数-alder 反应的隐形放射免疫治疗

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Química

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JoVE Journal Química

Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

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基于逆电子需求函数-alder 反应的隐形放射免疫治疗

DOI:

10.3791/59041-v

•

09:44 min

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January 29, 2019

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Rosemery Membreno², Brendon E. Cook^2,3, Brian M. Zeglis^2,3,4

¹Department of Chemistry,Hunter College of the City University of New York, ²Ph.D. Program in Chemistry,Graduate Center of the City University of New York, ³Department of Radiology,Memorial Sloan Kettering Cancer Center, ⁴Department of Radiology,Weill Cornell Medical College

Capítulos

00:04Título
00:41The Preparation of huA33-TCO
03:54¹⁷⁷Lu Radiolabeling of Tz-PEG₇-DOTA
05:10In vivo Studies
07:08Results: Longitudinal Therapy Study of Mice Bearing Subcutaneous SW1222 Tumors
09:00Conclusion

Summary

Tadução automática

该协议描述了反式环磷 (tco) 修饰抗体和^177个lu-tazine (tz) 辐射寡核苷酸的合成和表征, 用于预定目标放射治疗 (prit)。此外, 它还详细介绍了这两种结构在结直肠癌小鼠模型中的应用, 用于体内生物分布和纵向治疗研究。

Tags

Keywords: Pretargeted Radioimmunotherapy PRIT Inverse Electron Demand Diels-Alder Reaction Colorectal Cancer Xenograft Antibody Conjugation TCO-NHS HuA33 Antibody Size Exclusion Chromatography UV-vis Spectrophotometry

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