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使用自动活单元成像仪的简单迁移/入侵工作流

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A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

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使用自动活单元成像仪的简单迁移/入侵工作流

DOI:

10.3791/59042-v

•

09:17 min

•

February 02, 2019

•

Xiajie Zhang², Brianna C. Morten², Rodney J. Scott^2,3, Kelly A. Avery-Kiejda²

¹Medical Genetics,Hunter Medical Research Institute, ²Priority Research Centre for Cancer Research, Innovation and Translation, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine,University of Newcastle, ³Pathology North,John Hunter Hospital

Capítulos

00:04Título
00:51Optimal Migration Assay Seeding Density
02:49Migration and Invasion Assay Preparation
03:36Wound Scratch
05:35Wound Assay Imaging and Data Analysis
06:43Results: Representative Migration and Invasion Assay Analyses
08:22Conclusion

Summary

Tadução automática

目前的协议描述了一种实时研究癌症细胞迁移和入侵的综合方法, 为研究细胞迁移和形态提供了一个易于重现和省时的选择。

Tags

Cell Migration Cell Invasion Cancer Cell Mobility Metastasis Live-cell Imager Automated Imaging Cell Proliferation Extracellular Matrix Scratch Assay Cell Density Optimization Real-time Analysis

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