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人原发性前列腺组织培养的处理与评价

JoVE Journal
Bioengenharia

This content is Free Access.

JoVE Journal Bioengenharia

Handling and Assessment of Human Primary Prostate Organoid Culture

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人原发性前列腺组织培养的处理与评价

DOI:

10.3791/59051-v

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08:45 min

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January 17, 2019

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Tara McCray*¹, Zachary Richards*¹, Joseph Marsili, Gail S. Prins^2,3, Larisa Nonn³

¹Department of Pathology,University of Illinois at Chicago, ²Departments of Urology, Physiology, and Biophysics,University of Illinois at Chicago, ³University of Illinois Cancer Center

Capítulos

00:04Título
01:04Collection of Organoids from Matrix Gel
02:09Formalin Fixation and Paraffin Embedding of Organoids for Histological Endpoints
04:00Sectioning the Formalin Fixed Paraffin Embedded (FFPE) Histology Gel Blocks
05:04Whole-mounting of Organoids for Immunofluorescent Staining
06:35Results: Analysis of Human Primary Prostate Organoids
07:56Conclusion

Summary

Tadução automática

在这里, 我们提出了一个协议, 以指导人类的原发性前列腺有机体处理, 然后建议终点, 以评估表型。介绍了种子、培养维护、基质凝胶回收、形态定量、嵌入切片、ffpe 切片、全装染色及商业检测的应用。

Tags

Keywords: Prostate Organoids In Vitro Culture Primary Cells Matrix Gel Neutral Protease Histology Embedding Precision Medicine Disease Modeling

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