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Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens

DOI:
10.3791/59102-v

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07:42 min

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March 19, 2019

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, ,
1Department of Biology,Federico II University, 2Department of Molecular Medicine and Medical Biotechnology,University of Naples “Federico II”

Capítulos

  • 00:04Título
  • 00:39Adsorption Reaction
  • 01:29Direct Adsorption Efficiency Evaluation
  • 02:42Data Analysis
  • 03:43Indirect Adsorption Efficiency Evaluation
  • 06:20Results: Representative Direct and Indirect Adsorption Efficiency Analysis
  • 07:19Conclusion

Summary

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This protocol focuses on the use of bacterial spores as a "live" nanobiotechnological tool to adsorb heterologous molecules with various biological activities. The methods to measure the efficiency of adsorption are also shown.

Tags

Keywords: Spore AdsorptionNonrecombinant Display SystemEnzymesAntigensMucosal VaccinesBiocatalystsB.subtilisRFPWestern BlotFluorescence MicroscopyImageJ

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