É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Título
- 01:05Conditioned Medium Collection and Processing
- 02:45Nanoparticle Tracking Analysis (NTA) Quantification
- 03:27Sample Preparation
- 04:17Sample Acquisition and Analysis
- 06:32Results: Representative Flow Cytometric Analyses of Extracellular Vesicles from Cell-conditioned Medium
- 07:58Conclusion
The protocol describes a reproducible method designed for use with cell culture supernatants to detect surface epitopes on small extracellular vesicles (EV). It utilizes specific EV immunoprecipitation using beads coupled with antibodies that recognize surface antigen CD9, CD63, and CD81. The method is optimized for downstream flow cytometry analysis.
