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High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
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High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils

High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils

DOI:
10.3791/59137-v

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12:36 min

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February 09, 2019

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, , , ,
1Department of Crop and Soil Sciences,Washington State University, 2Department of Plant Pathology,Washington State University

Capítulos

  • 00:04Título
  • 01:01Preparation of Chrome Azurol S (CAS)-Fe-Agar Medium
  • 03:35Pyoverdine/EDTA Standard Preparation
  • 05:04Collection of Environmental Samples: Soil and Plant Tissues
  • 06:38Preparation of Siderophore Enrichment Cultures and CAS-Fe Siderophore Production Assay
  • 08:11CAS-Fe Agar Assays for Detection of Siderophore Production in Environmental Samples
  • 09:34Results: Pyoverdine Equivalents of Siderophore Enrichment Cultures
  • 11:28Conclusion

Summary

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We present a protocol for rapid screening of environmental samples for siderophore potential contributing to micronutrient bioavailability and turnover in terrestrial systems.

Tags

Siderophore ScreeningHigh-throughputEnvironmental SamplesBulk SoilsRhizosphere SoilsIron CyclingMicrobial CommunityAgriculture ProductivityFarm Management PracticesSoil HealthCAS AssayHDTMACAS SolutionPIPES Buffer SolutionAgarose

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