Journal
/
Pesquisa do Câncer
/
Extraction of Extracellular Vesicles from Whole Tissue
JoVE Journal
Pesquisa do Câncer
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Pesquisa do Câncer
Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

DOI:
10.3791/59143-v

•

09:03 min

•

February 07, 2019

•

, ,
1Department of Biomedical Sciences,Florida State University College of Medicine

Capítulos

  • 00:04Título
  • 00:51Tissue Dissociation and Differential Centrifugation
  • 03:15Density Gradient Purification
  • 05:53Lysis and Immunoblot Confirmation of EV Proteins
  • 07:10Results: Analysis of Extracted Extracellular Vesicles
  • 08:09Conclusion

Summary

Tadução automática

Tadução automática

Here, we provide a detailed protocol to isolate small extracellular vesicles (EVs) from whole tissues, including brain and tumor specimens. This method offers a reproducible technique to extract EVs from solid tissue for further downstream analyses.

Tags

Extracellular VesiclesUltracentrifugationEV IsolationTumor SpecimensProtease InhibitorsCentrifugationFiltrationResuspension

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59143?article_type=v

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
check_url/pt/59143?article_type=v

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels
check_url/pt/59143?article_type=v

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
check_url/pt/59143?article_type=v

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
check_url/pt/59143?article_type=v

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
check_url/pt/59143?article_type=v

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
check_url/pt/59143?article_type=v

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
check_url/pt/59143?article_type=v

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
check_url/pt/59143?article_type=v

Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
check_url/pt/59143?article_type=v

Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

Read Article