Journal
/
Biologia
/
一种用于阴南芥叶片安装的通用方法
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging
Please note that all translations are automatically generated. Click here for the English version.

一种用于阴南芥叶片安装的通用方法

DOI:
10.3791/59147-v

•

07:52 min

•

February 11, 2019

•

, ,
1Japan Science and Technology Agency (JST), PRESTO, 2Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 3Faculty of Life and Environmental Sciences,University of Tsukuba, 4Microbiology Research Center for Sustainability,University of Tsukuba

Capítulos

  • 00:04Título
  • 00:28Preparation of Plants and Pathogens
  • 01:48Pathogen Inoculation
  • 03:10Mounting the Inoculated Leaf
  • 05:02Microscopic Time-lapse Observation
  • 06:19Results: Intravital Time-lapse Imaging of Mounted Arabidopsis Leaves
  • 07:16Conclusion

Summary

Tadução automática

Tadução automática

我们报告了一种简单和通用的方法, 用于在较长时间内对拟南芥叶片进行荧光实时成像。我们使用转基因拟南芥植物表达荧光记者基因在免疫相关的启动子控制下作为一个例子, 以获得时空了解植物免疫反应。

Tags

Arabidopsis LeavesIntravital Time-lapse ImagingSpatiotemporal RegulationGene Of InterestImmunityPromoter ActivityPathogen InoculationBacterial SuspensionFluorescence StereomicroscopePR1 Promoter

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

有丝分裂的siRNA转染后的时间推移成像

冰帽：为发展方法拟南芥和番茄植株在96孔板高通量的基因分型

一个影像的简单方法拟南芥叶浸润成像介质中使用全氟萘烷

从长期来看，高解析度激光共聚焦时间流逝成像<em>拟南芥子叶</em>表皮萌发过程中

时间推移视频显微镜对EYFP-Parkin的聚集性评估作为蜂窝Mitophagy一个标记

高分辨率成像定时，并在活体人脐静脉内皮细胞微管动力学自动分析

拟南芥种子高通量灭菌的标准化方法

延时结构照明显微镜线粒体核素的特定标签

LarvaSPA，一种用于长期延时成像的安装果蝇幼虫的方法

细菌群的延时成像与集体压力反应

Read Article