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分离叶测定,以简化马铃薯中基因表达研究,在咀嚼昆虫曼杜卡性塔感染

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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

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分离叶测定,以简化马铃薯中基因表达研究,在咀嚼昆虫曼杜卡性塔感染

DOI:

10.3791/59153-v

•

05:56 min

•

May 15, 2019

•

Nicole G. Novak, Frances G. Perez, Richard W. Jones, Susan D. Lawrence

¹Invasive Insect Biocontrol and Behavior Lab,United States Department of Agriculture (USDA), Agricultural Research Service (ARS), ²Genetic Improvement for Fruits and Vegetables Lab,USDA-ARS

Capítulos

00:04Título
01:02Potato Plant and Insect Preparation
02:02Infestation Experiment Setup
03:14Infestation and Leaf Harvest
04:16Results: Representative Leaf Damage and Gene Expression
05:23Conclusion

Summary

Tadução automática

该方法通过将曼杜卡性幼虫应用于马铃薯分离叶,创造出天然的草食性植物组织受损。植物组织被测定为六个转录因子同源的表达参与早期反应昆虫食草动物。

Tags

Detached Leaf Assay Gene Expression Potato Manduca Sexta Insect Herbivory Chewing Insect Nodal Propagation Larval Stage Plant-insect Interaction Reproducible Data Plant Genetic Improvement

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