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Aussaat und Implantation von einem biosynthetischen Tissue-Engineering trachealen Transplantat in einem Mausmodell
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1Department of Otolaryngology Head & Neck Surgery,Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine,Research Institute at Nationwide Children’s Hospital, 4Center for Perinatal Research,Nationwide Children’s Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery,Nationwide Children’s Hospital
Capítulos
- 00:04Título
- 01:15Scaffold Manufacturing
- 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
- 04:40Cell Seeding
- 05:21Graft Implantation
- 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
- 09:27Conclusion
Transplantat Stenose stellt eine kritische Hindernis Gewebezüchtungen Atemwege Ersatz. Um zellulären Mechanismen, die Stenose zu untersuchen, verwenden wir ein Mausmodell entwickelt trachealen Gewebeersatz mit kernigen Knochenmark mononukleären Zellen (BM-MNC). Hier zeigen wir unsere Protokoll, einschließlich Gerüst Herstellung, BM-MNC Isolierung Transplantat Aussaat und Implantation.
