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Brug af Nanoplasmon-forbedret spredning og lav forstørrelse Microscope Imaging til at kvantificere tumor afledte Exosomer
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1Department of Integrated Traditional Chinese and Western Medicine,West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute,Arizona State University, 3School of Biological and Health Systems Engineering,Arizona State University, 4Department of Electrical and Computer Engineering,North Dakota State University
Capítulos
- 00:04Título
- 01:01Preparation of Nanoparticle Probes (AuNR-IgG)
- 02:17Preparation of Extracellular Vesicle Capture Slides and Exosome Capture
- 05:17Low-Magnification Dark-Field Microscope (LMDFM) Image Acquisition and Analysis
- 07:48Results: Exosome Quantification by Analysis of LMDFM Images
- 08:25Conclusion
Klinisk oversættelse af exosome afledte biomarkører for syge og maligne celler hæmmes af manglen på hurtige og præcise kvantificeringsmetoder. Denne rapport beskriver brugen af mørke mikroskoper med lav forstørrelse til at kvantificere specifikke exosomvesikler subtyper i små mængder serum-eller plasmaprøver.
