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线虫的核苷酸和蛋白质提取联合
JoVE Journal
Biologia
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JoVE Journal Biologia
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
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线虫的核苷酸和蛋白质提取联合

DOI:
10.3791/59178-v

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10:37 min

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March 17, 2019

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, , ,
1Department of Molecular Biology, Cell Biology and Biochemistry,Brown University

Capítulos

  • 00:04Título
  • 00:33Sample Collection
  • 01:31Nucleotide and Protein Isolation
  • 07:01Evaluating mRNA with RT-qPCR
  • 08:12Results: Extractions of Different Macromolecules from the Same C. elegans Sample
  • 09:40Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个从同一样本中分离 RNA、DNA 和蛋白质的协议, 以减少变异, 提高重现性, 并促进解释。

Tags

Keywords: Caenorhabditis ElegansNucleotide ExtractionProtein ExtractionMRNAMacromoleculesSample VariabilityReproducibilitySample CollectionCross-sectional AnalysisWormsM9 BufferGTCP ReagentChloroformRNA IsolationIsopropanolEthanolDNA IsolationProtein Isolation

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