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Capítulos
- 00:04Título
- 01:05Non-malignant B and T Lymphocyte Isolation from Transgenic lck:eGFP Fish
- 02:11Malignant Lymphocyte Isolation from Double-transgenic rag2:hMYC;lck:eGFP Zebrafish
- 04:55Normal or Malignant B Lymphocyte Flow Cytometric Analysis
- 05:50Results: Representative Malignant and Non-malignant B Cell GFP Expression in Ick:eGFP Zebrafish
- 07:44Conclusion
Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP zebrafish.
