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研究病原体或药物交叉障碍及其与大脑相互作用的人血脑界面模型
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Neurociência
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JoVE Journal Neurociência
A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain
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研究病原体或药物交叉障碍及其与大脑相互作用的人血脑界面模型

DOI:
10.3791/59220-v

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07:52 min

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April 09, 2019

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, , , , , ,
1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS

Capítulos

  • 00:04Título
  • 00:36Human Cerebral Microvascular Endothelial Cell (hCMEC)/D3 Insert Coating and Blood Brain Barrier (BBB) Preparation
  • 02:48BBB-Minibrain Endothelial Permeability Validation
  • 03:38BBB Crossing and Multiplication of Yellow Fever Viruses in the Minibrain and Biomolecule BBB Crossing and Brain Cell Targeting
  • 05:22Results: Representative BBB-Minibrain Testing of Neuroinvasive Variants and Biomolecule Properties
  • 07:19Conclusion

Summary

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在这里, 我们提出了一个协议, 描述了设置在纤维素 BBB (血脑屏障)-minibrain 聚酯多孔膜培养插入系统, 以评估生物分子或传染因子在人类 BBB 的运输和对邻近脑细胞的生理影响。

Tags

Blood-brain InterfaceBlood-brain BarrierBBBPathogenMedicinaNeurosystemMini-brainEndothelial CellsLucifer YellowPermeabilityTransport BufferCHME Clone Five CellsNeuronsAstrocytesPolyester MembraneCell CultureCell SeedingCell CountingCell Trypsinization

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