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Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
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Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

DOI:
10.3791/59223-v

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09:28 min

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May 12, 2019

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, , , , , , , , , ,
1Laboratory of Translational Cancer Research, Institute for Translational Research,Ochsner Clinic Foundation, 2Department of Urology,Ochsner Clinic Foundation, 3Department of Colon and Rectal Surgery,Ochsner Clinic Foundation, 4Ochsner Clinical School,University of Queensland, School of Medicine

Capítulos

  • 00:04Título
  • 00:55Tumor Enzymatic Digestion
  • 02:54UCC Mouse Model
  • 04:48CRC Mouse Model
  • 06:02Bioluminescent Imaging, Organ Harvesting and Evaluation
  • 06:55Results: Xenograft Tumor Analysis and Comparison with Primary Patient Tumors
  • 08:44Conclusion

Summary

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This protocol describes the generation of patient-derived orthotopic xenograft models by intra-vesically instilling high-grade urothelial cell carcinoma cells or intra-rectally injecting colorectal cancer cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice for primary tumor growth and spontaneous metastases under the influence of lymph node stromal cells, which mimics the progression of human metastatic diseases.

Tags

Keywords: Orthotopic XenograftUrothelial Cell CarcinomaColorectal CancerTumor GrowthSpontaneous MetastasisLymph NodeTumor ImplantationMetastatic PatternsLuciferaseCollagenaseHyaluronidaseDeoxyribonucleaseCell StrainerNOD-SCID MouseElectrocautery

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