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趋化梯度多细胞响应的三维分析

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

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趋化梯度多细胞响应的三维分析

DOI:

10.3791/59226-v

•

05:57 min

•

May 24, 2019

•

Tae-Yun Kang, David Ellison, Sung Hoon Lee, Andrew J. Ewald³, Andre Levchenko

¹Department of Biomedical Engineering and Yale Systems Biology Institute,Yale University, ²Department of Biomedical Engineering,Johns Hopkins University, ³Center for Cell Dynamics and Department of Cell Biology,Johns Hopkins University

Capítulos

00:04Título
00:34Mesofluidic Device Fabrication
02:20Collagen Injection, 3D Imaging, and Quantification
04:20Results: Representative Ligand Gradient Evaluation and Cellular Gradient Reponses
05:23Conclusion

Summary

Tadução automática

我们描述了一种方法来构建设备的三维培养和实验与细胞和多细胞有机体。该装置允许分析在具有明确趋化因子梯度的三维微环境中对可溶性信号的细胞反应。在检测弱噪声输入时, 有机体优于单个细胞。

Tags

3D Analysis Multi-cellular Response Chemoattractant Gradients PDMS Device Mesofluidic Device Stereo Lithography Vacuum Degassing PDMS Curing Cell Loading Collagen Cell Suspension Cell Culture 3D Physiological Environment

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