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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
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Neurociência
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JoVE Journal Neurociência
Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

DOI:
10.3791/59232-v

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08:52 min

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May 23, 2019

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, , ,
1Departments of Bioengineering,University of Washington, 2Washington National Primate Research Center, 3Department of Electrical and Computer Engineering,University of Washington, 4Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

Capítulos

  • 00:04Título
  • 00:39MR-compatible Chamber Implantation
  • 02:05Viral Vector Delivery
  • 06:18Results: Validation of Viral Expression
  • 07:47Conclusion

Summary

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Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

Tags

Keywords: Convection Enhanced DeliveryOptogeneticAdeno-associated Viral VectorCortexRhesus MacaqueMRICraniotomyDuraMR Compatible ChamberInjection GridSalineSterile Absorbable Gel FoamVitamin E Capsule

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