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1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine,University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine,University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine,University of California, Los Angeles
Capítulos
- 00:04Título
- 00:40Vector Design and Generation
- 01:52Virus Generation
- 04:24Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
- 07:07dCas9 and gRNA Transduction, Selection, and Clone Creation
- 10:18Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
- 12:08Conclusion
Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.
