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使用小鼠化疗引起的粘炎模型评估小肠损伤和适应的重要端点和增殖标记

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Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis

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使用小鼠化疗引起的粘炎模型评估小肠损伤和适应的重要端点和增殖标记

DOI:

10.3791/59236-v

•

07:05 min

•

May 12, 2019

•

Anna Billeschou, Jenna Hunt, Hannelouise Kissow²

¹Department of Biomedical Sciences, Faculty of Health and Medical Sciences,University of Copenhagen, ²Novo Nordisk Foundation Center of Basic Metabolic Research, Faculty of Health and Medical Sciences,University of Copenhagen

Capítulos

00:04Título
00:325-Fluorouracil (5-FU) Mucositis Induction
01:095-Bromo-2 -Deoxyuridine (BrdU) Quantification and Tissue Collectio
02:42BrdU Immunoreactive Cell Area Measurement
04:23Crypt Depth Measurement
04:57Results: Representative Small Intestine Injury Recovery Assessment
06:17Conclusion

Summary

Tadução automática

在这里,我们提出了一个协议,建立重要的终点和小肠损伤和补偿性增殖,使用化疗引起的粘炎模型。我们演示了使用细胞周期特定标记和使用小肠道重量、墓穴深度和绒痛高度作为端点来检测增殖细胞。

Tags

5-Florauracil 5FU Chemotherapy-induced Mucositis Mouse Model Small Intestinal Injury Compensatory Hyperproliferation BrdU Quantification Histological Processing Image Analysis

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