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דיסקציה של Ca מקומי2 + אותות בתאים בתרבית על ידי קרום, ממוקדות Ca2 + אינדיקטורים
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1Japan Science and Technology Agency,PRESTO, 2Laboratory for Developmental Neurobiology,RIKEN Center for Brain Science, 3École Normale Supèrieure, Institut de Biologie de l’ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University
Capítulos
- 00:04Título
- 01:10Preparation of Poly(ethyleneimine) Coated Coverslips
- 02:42Preparation of Rat Cortical Neuron-astrocyte Mixed Culture and Frozen Cells, and the Revival of Frozen Cultures
- 05:45Transfection and Adeno-associated Virus Infection of Hippocampal or Cortical Neurons
- 07:29Recording Spontaneous Activities of Astrocytes Expressing Lck-RCaMP2 and OER-GCaMP6f
- 08:37Results: Dissection of Local Ca2+ Signals in Cultured Cells
- 10:38Conclusion
