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Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips
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Capítulos
- 00:04Título
- 00:45Preparation of the Multi-compartment Chips
- 01:05Coating of Multi-compartment Chips for hSC-neurons
- 02:59Seeding NSCs into the Multi-compartment Chip
- 04:05Viral Fluorescent Labeling of hSC-neurons within the Chip
- 05:02Results: Using Compartmentalized Microfluidic Chips to Isolate Neurons Differentiated from Human Stem Cells
- 06:16Conclusion
This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining.
