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预组装塑料微流体芯片中人类干细胞衍生神经元的分段化

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

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预组装塑料微流体芯片中人类干细胞衍生神经元的分段化

DOI:

10.3791/59250-v

•

06:46 min

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May 03, 2019

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Smita R. Paranjape², Tharkika Nagendran², Valerie Poole, Joseph Harris, Anne Marion Taylor^2,3

¹UNC Neuroscience Center, ²UNC/NC State Joint Department of Biomedical Engineering, ³Xona Microfluidics, LLC

Capítulos

00:04Título
00:45Preparation of the Multi-compartment Chips
01:05Coating of Multi-compartment Chips for hSC-neurons
02:59Seeding NSCs into the Multi-compartment Chip
04:05Viral Fluorescent Labeling of hSC-neurons within the Chip
05:02Results: Using Compartmentalized Microfluidic Chips to Isolate Neurons Differentiated from Human Stem Cells
06:16Conclusion

Summary

Tadução automática

该协议演示了使用分门分割的微流体芯片,注射成型在循环烯烃共聚物培养的神经元区别于人类干细胞。这些芯片是预先组装的,比传统的分块聚(二甲基硅氧烷)器件更容易使用。这里描述了多种常见的实验模式,包括病毒标记、流体隔离、轴切除术和免疫染色。

Tags

Keywords: Compartmentalized Microfluidic Devices Human Stem Cell-derived Neurons COC Chips PDMS Multi-compartment Devices Poly-L-ornithine Laminin Neural Stem Cells Differentiation Neuroscience Research Cell Culture

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