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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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JoVE Journal Pesquisa do Câncer
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

DOI:
10.3791/59271-v

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08:07 min

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April 12, 2019

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, ,
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences,Sun Yat-sen University

Capítulos

  • 00:04Título
  • 00:59Bone Preparation and Demineralization
  • 02:29Bone Decellularization
  • 04:08Cell Seeding and Culture
  • 06:26Results: Characterization of Decellularized BEM and OS Cells
  • 07:44Conclusion

Summary

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The bone extracellular matrix (BEM) model for osteosarcoma (OS) is well established and shown here. It can be used as a suitable scaffold for mimicking primary tumor growth in vitro and providing an ideal model for studying the histologic and cytogenic heterogeneity of OS.

Tags

3D Bone Extracellular MatrixOsteosarcomaDecellularizationBone-derived MatrixBone Tumor ResearchHeterogenous MorphologyDrug SensitivityEwing SarcomaMetastatic Bone TumorsBALB/c MiceBone DecalcificationLipid Extraction

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