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In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

DOI:
10.3791/59275-v

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08:04 min

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February 27, 2019

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, , , , ,
1Department of Hematology & Hematopoietic Cell Transplantation, T Cell Therapeutics Research Laboratory,City of Hope Beckman Research Institute and Medical Center, 2Irell and Manella Graduate School of Biological Sciences,City of Hope Beckman Research Institute and Medical Center

Capítulos

  • 00:04Título
  • 00:54Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge
  • 02:37Sample Harvest for Flow Cytometric Analysis
  • 04:12Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis
  • 05:14Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge
  • 07:30Conclusion

Summary

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Here, we describe an in vitro co-culture method to recursively challenge tumor-targeted T cells, which allows for phenotypic and functional analysis of antitumor T cell activity.

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Keywords: In VitroTumor CellRechallengeChimeric Antigen ReceptorCAR T CellsAnti-tumor FunctionGlioblastomaTumorspheresCo-cultureHigh Throughput ScreeningCell DissociationCell SuspensionCell CultureSupernatantTrypsin EDTA

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