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Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
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Capítulos
- 00:04Título
- 00:37Surgical Preparation
- 02:16Tumor Cell Injection and Cranial Imaging Window (CIW) Preparation
- 03:26Intravital Imaging Setup
- 04:07Time-Lapse Image Acquisition
- 05:02Biopsy-Like Injury and CIW Replacement
- 05:50Image Analysis
- 06:56Results: Representative Impact of Biopsy on Tumor Cell Migration
- 08:56Conclusion
Here we describe a method for high-resolution time-lapse multiphoton imaging of brain tumor cells before and after invasive surgical intervention (e.g., biopsy) within the same living animal. This method allows studying the impact of these invasive surgical procedures on tumor cells' migratory, invasive, and proliferative behavior at a single cell level.
