Journal
/
Neurociência
/
富德瑞迪蒂菲洛波迪亚成分的纯化
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Purification of the Dendritic Filopodia-rich Fraction
Please note that all translations are automatically generated. Click here for the English version.

富德瑞迪蒂菲洛波迪亚成分的纯化

DOI:
10.3791/59292-v

•

11:51 min

•

May 02, 2019

•

,
1Laboratory for Neurobiology of Synapse,RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit,RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology,RIKEN Center for Brain Science

Capítulos

  • 00:04Título
  • 00:35Preparation of Culture Medium and Poly-L-Lysine-coated Dishes
  • 04:58Preparation of Hippocampal Neurons
  • 07:04Purification of Dendritic Filopodia-rich Fraction, Silver Staining and Western Blot Analysis
  • 09:33Results: Purification Method for the Dendritic Filopodia-rich Fraction
  • 11:12Conclusion

Summary

Tadução automática

Tadução automática

在此协议中,我们介绍了一种利用树突状纤维蛋白之间特定且强烈的亲和力,从培养的海马神经元上的方皮杯状突起结构中纯化树突状纤维素的富足部分的方法。粘附分子,TLCN,和细胞外基质分子,体外内丁。

Tags

Dendritic FilopodiaSynaptic ProteinImmature SynapsePhagocytic Cap FormationArtificial GenesisVitamin MixRiboflavin SolutionCalcium DichlorideMinimum Essential Medium (MEM)DNase-1Ara-CPlating Medium

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59292?article_type=v

在培养的中枢神经系统的神经元树突棘形态分析
check_url/pt/59292?article_type=v

诱导培养的交感神经元的树突状增长
check_url/pt/59292?article_type=v

浦肯野细胞树突形态的器官切片培养分析
check_url/pt/59292?article_type=v

谷氨酸和GABA快速微离子导入：一个有用​​的工具，调查突触整合
check_url/pt/59292?article_type=v

树突分支中的海马区对小鼠的齿状回评
check_url/pt/59292?article_type=v

对小鼠脑血管净化
check_url/pt/59292?article_type=v

分析树突状形态的列和图层
check_url/pt/59292?article_type=v

使用高尔基Cox方法评估老年小鼠海马树突状复杂性
check_url/pt/59292?article_type=v

用四甲基铵进行实时离子电渗疗法，以量化体细胞分数和脑细胞外空间的变性
check_url/pt/59292?article_type=v

嗜德维人神经元树突状树突状复杂度的定量分析

Read Article