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Biologia do Desenvolvimento
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Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

DOI:
10.3791/59299-v

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08:47 min

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March 15, 2019

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,
1Department of Biology,Wesleyan University

Capítulos

  • 00:04Título
  • 00:29Eye-Brain Complex Dissection
  • 02:09Tissue Processing for Immunofluorescence
  • 04:56Tissue Processing for Western Blotting
  • 06:07Tissue Processing for RNA Extraction
  • 07:07Results: Representative Pupal Eye Protein and Gene Expression Analyses
  • 08:28Conclusion

Summary

Tadução automática

Tadução automática

This paper presents a surgical method for dissecting Drosophila pupal retinas along with protocols for the processing of tissue for immunohistochemistry, western analysis, and RNA-extraction. 

Tags

Keywords: DrosophilaPupal RetinaImmunohistochemistryWestern AnalysisRNA IsolationDissectionEye-brain ComplexMicrodissectionForcepsPBSFixative

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