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Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure
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Bioengenharia
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JoVE Journal Bioengenharia
Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure

Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure

DOI:
10.3791/59318-v

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10:26 min

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May 06, 2019

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, , ,
1Department of Biology,Utah State University

Capítulos

  • 00:04Título
  • 02:21Recombinant Spider Silk Mixture Preparation from Lyophilized Protein Stocks
  • 03:03Recombinant Spider Silk Solvation
  • 04:05Hydrogels, Sponges, and Lyogel Preparation
  • 04:55Films, Coatings and Adhesives
  • 06:01Wet-Spun Fibers
  • 09:04Results: Aqueous Solvation and rSSp Materials
  • 09:38Conclusion

Summary

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Here, we present a protocol to produce water soluble recombinant spider silk protein solutions and the material forms that can be formed from those solutions.

Tags

Recombinant Spider SilkAqueous SolvationHeatPressureProtein SolubilizationMaterial FormationWater-based ProcessProtein Structure-functionInsoluble ProteinsMicrowaveSalt Removal

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