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Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
JoVE Journal
Biologia
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JoVE Journal Biologia
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
DOI:

00:06 min

August 20, 2019

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Capítulos

  • 00:04Título
  • 00:35Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
  • 02:47Pretreatment, Immunostaining and Mounting of the Aorta
  • 04:37Results: Representative En Face Staining Images
  • 05:54Conclusion

Summary

Tadução automática

Here, we present a protocol for immunofluorescence staining to observe the endothelial cells of the mouse aorta directly. This technique is useful when studying the cellular and molecular phenotype of endothelial cells in different flow patterns and in the development of atherosclerosis.

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