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Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
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Capítulos
- 00:04Título
- 01:12Preparation of Keratinocyte Culture Medium and the Fibril Form of Type I Collagen
- 02:53Preparation of the Non-fibrous Form of Type I Collagen
- 03:50Culture of FEPE1L-8 Cells
- 05:20Estimation of the Number of Viable Cells
- 06:20Results: Evaluation of Keratinocyte Proliferation
- 07:01Conclusion
Here, we present a protocol for the preparation of two different forms of culture substrates utilizing type I collagen. Depending on how collagen is handled, collagen molecules either maintain two-dimensional, non-fibrous form or reassemble into three-dimensional, fibril form. Cell proliferation on type I collagen is drastically affected by fibril formation.
