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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
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JoVE Journal Bioquímica
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

DOI:
10.3791/59341-v

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04:18 min

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March 31, 2019

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, ,
1Department of Molecular Biology and Chemistry,Christopher Newport University

Capítulos

  • 00:04Título
  • 00:29Preparing the Gel
  • 01:29Loading and Running the Gel
  • 02:20Visualization of Thiazole Orange Agarose Gel
  • 03:08Image Capture
  • 03:28Results: DNA Staining with Thiazole Orange in Electrophoresis Experiments
  • 03:58Conclusion

Summary

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Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.

Tags

Keywords: DNA ElectrophoresisThiazole OrangeEthidium BromideAlternative DyesUV LightBlue LightAgarose GelTAE BufferTBE BufferDNA DetectionDNA VisualizationDNA ExtractionDownstream Experiments

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