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CRISPR/Cas12a 多路复用基因组编辑糖母细胞和酵母像素艺术的创作

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

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CRISPR/Cas12a 多路复用基因组编辑糖母细胞和酵母像素艺术的创作

DOI:

10.3791/59350-v

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10:18 min

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May 28, 2019

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Klaudia Ciurkot², Brenda Vonk, Thomas E. Gorochowski⁴, Johannes A. Roubos, René Verwaal

¹DSM Biotechnology Center, ²Biochemistry and Molecular Biology, Department of Chemistry,University of Hamburg, ³BrisSynBio,University of Bristol, Life Sciences Building, ⁴School of Biological Sciences,University of Bristol, Life Sciences Building

Capítulos

00:04Título
01:22Single CRISPR RNA (crRNA) Array Expression Cassette
02:10S. cerevisiae Transformation
05:02Second Transformation
06:42Genome Editing Efficiency Evaluation and Yeast Pixel Art Creation
08:30Results: Transformation Verification and Yeast Pixel Art Creation
09:39Conclusion

Summary

Tadução automática

CRISPR/Cas12a 系统与单个 crRNA 阵列相结合,可同时在多个位点对S. cerevisae基因组进行高效的多路复用编辑。这通过构建产生酵母菌株的类胡萝卜素来证明,这些菌株随后用于创建酵母像素艺术。

Tags

CRISPR/Cas12a Multiplex Genome Editing Saccharomyces Cerevisiae Yeast Pixel Art Carotenoids CrRNA Array Cas12a In Vivo Recombination PCR Amplification Yeast Transformation YEPD Medium Lithium Acetate Plasmid PCSN067

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