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单分子跟踪显微镜 - 确定细胞分子扩散状态的工具
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Bioquímica
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JoVE Journal Bioquímica
Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
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单分子跟踪显微镜 - 确定细胞分子扩散状态的工具

DOI:
10.3791/59387-v

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00:10 min

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September 05, 2019

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,
1Department of Chemistry,University of Virginia, 2Department of Molecular Physiology & Biological Physics,University of Virginia School of Medicine

Capítulos

  • 00:04Título
  • 00:35Microscope Preparation
  • 02:33Y. enterocolitica Culture Preparation
  • 03:33Data Acquisition
  • 05:54Data Processing
  • 06:45Data Post-processing – Overlay of Outlines
  • 07:32Results: Determining the Diffusive States of YscQ
  • 09:25Conclusion

Summary

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Tadução automática

3D 单分子定位显微镜用于探测活细菌细胞中荧光标记蛋白质的空间位置和运动轨迹。本文描述的实验和数据分析方案根据集合的单分子轨迹确定细胞蛋白的普遍扩散行为。

Tags

Keywords: Single-molecule Tracking3D TrackingDiffusive StatesCytosolic MoleculesFluorescence MicroscopyAgarose PadYersinia EnterocoliticaCell VolumeZ-calibrationMATLABHCImage Live

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