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1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,University of Notre Dame, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,University of Notre Dame, 5Harper Cancer Research Institute,University of Notre Dame, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
Capítulos
- 00:04Título
- 00:32Nitro-Angiotensin II Desalting
- 01:30Primary Amine Alkylation by Dimethyl Labeling
- 02:37Nitrotyrosine to Aminotyrosine Reduction and Biotinylation, Enrichment, and Detection
- 06:03Results: Representative Mass Spectra Analyses
- 06:59Conclusion
Proteomisk profilering av tyrosin-Nitrerade proteiner har varit en utmanande teknik på grund av det låga överflöd av 3-nitrotyrosin modifiering. Här beskriver vi en ny metod för nitropeptid berikning och profilering genom att använda angiotensin II som modell. Denna metod kan för längas för andra in vitro -eller in vivo -system.
