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斑马鱼手术尺寸缩小术中胚胎形态缩放的研究
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling
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斑马鱼手术尺寸缩小术中胚胎形态缩放的研究

DOI:
10.3791/59434-v

•

06:31 min

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May 03, 2019

•

, , ,
1Department of Systems Biology,Harvard Medical School

Capítulos

  • 00:04Título
  • 01:16Tool Preparation
  • 02:08Surgical Size Reduction and Recovery
  • 04:03Results: Surgical Size Reduction Leads to Changes in Morphology, Somite Size, and Neural Tube Heights
  • 05:24Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们描述了一种在不破坏正常发育过程的情况下减小斑马鱼胚胎大小的方法。这种技术可以研究模式缩放和针对大小变化的开发鲁棒性。

Tags

ZebrafishEmbryonic Pattern ScalingSize ReductionLive ImagingGenéticaQuantitative AnalysisChorion RemovalWire LoopMethyl CelluloseRinger’s SolutionBlastulaAnimal Pole

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