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利用标准化定量西方印迹技术, 对跨组织和整个开发过程中的蛋白质水平进行可靠比较

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

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利用标准化定量西方印迹技术, 对跨组织和整个开发过程中的蛋白质水平进行可靠比较

DOI:

10.3791/59438-v

•

08:13 min

•

April 09, 2019

•

Yu-Ting Huang², Dinja van der Hoorn*^1,2, Leire M. Ledahawsky*^1,2, Anna A. L. Motyl*^1,2, Crispin Y. Jordan, Thomas H. Gillingwater², Ewout J. N. Groen²

¹Centre for Discovery Brain Sciences,University of Edinburgh, ²Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh

Capítulos

00:04Título
00:34Protein Extraction
01:15Quantification and Normalization of Protein Concentration
02:22Protein Sample Gel Electrophoresis and Protein Transfer
04:03Total Protein Staining
05:26Image Acquisition and Western Blot Analysis
06:39Resultados
07:32Conclusion

Summary

Tadução automática

该方法描述了一种可靠且可重复的方法, 用于使用标准化的定量西方印迹法比较不同组织和不同发育时间点的蛋白质水平。

Tags

Keywords: Protein Levels Quantitative Western Blotting Standardized Protocol Developmental Analysis Fluorescent Total Protein Stain Internal Loading Control Protein Extraction Protein Quantification Electrophoresis Gel Loading Between-membrane Normalization

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